Abstract
Live imaging techniques using fluorescent tags and fluorescent tracers have enabled the detection of the organelles and vesicles. However, the ultrastructure and organelle distribution such as Golgi vesicle and endosome are not clearly detectable in the plant cell. Therefore, we are performing an exhaustive ultrastructural analysis of organelles in Arabidopsis root and shoot apex using high-pressure freezing and electron microscopic techniques. Arabidopsis seedlings were frozen in high-pressure freezer, and the samples were fixed and embedded in the resin. The ultra-thin sections were widely taken pictures by TEM. Then the merged pictures were transformed to annotate the location data of organelles. Using the mapping system we observed highly dense large vesicles derived from the Golgi and also ER bodies in root apex cells but not in shoot apex cells. Meanwhile, many clusters of vesicles were existed in shoot cells but hardly in root cells. We are now analyzing about distribution of other organelles in the root and shoot apex.
