Molecular biological analysis of polyamine oxidase genes in Arabidopsis thaliana and Oryza sativa

Abstract

Phylogenetic analysis on plant polyamine oxidases (PAOs) revealed that they are categorized into 4 clades. The model plants Arabidopsis thaliana and Oryza sativa contain 5 and 7 PAO members, respectively. AtPAO5 and OsPAO1 belong to one clade while AtPAO1 builts a second clade with tobacco NtPAO and apple MdPAO. OsPAO2, OsPAO6 and OsPAO7 form the third clade, to which maize ZmPAO1 belongs. AtPAO2, AtPAO3, AtPAO4, OsPAO3, OsPAO4 and OsPAO5 belong to the forth one. Here we have examined the tissue-specific and space-temporal expression of the AtPAO members. Recombinant AtPAO proteins were produced in Escherichia coli and their substrate specificity was demonstrated. The tissue-specific expression of the rice OsPAO members in seedling and flowering stages was also addressed. We will refer to the substrate specificity of OsPAO proteins.