Abstract
Plants undergo continuous exposure to various biotic and abiotic stresses in their natural environment. These stresses often cause accumulation of reactive oxygen species (ROS), leading to the development of oxidative stresses. To understand the crosstalk in biotic and abiotic stress signaling, we focused on analysis of oxidative stress response. Methyl viologen (MV) is a herbicide that catalyze the formation of superoxide free radical, a major ROS.
We found an ecotype Nos-d to show resistance to methyl viologen. The Nos-d also showed tolerance to osmotic stress, but not to salinity or stress related hormones. Nos-d also showed tolerance to another oxidative stress-related chemicals, but the difference between Nos-d and Col was very slight. To isolate another MV-tolerant accessions, MV tolerance of Arabidopsis accessions distributed from RIKEN BRC was evaluated. Several MV tolerant accessions were identified. Analysis using Nos-d x Col F2 population suggested that the resistant phenotype is linked to a single locus. Mapping of the locus of MV tolerance is now in progress to understand the tolerance mechanism.
