Abstract
In Arabidopsis, flavonoid biosynthesis is regulated by MYB transcription factors such as MYB12 for flavonol and MYB75/PAP1 for anthocyanin. We have generated the transgenic lines over-expressing both MYB12 and PAP1 (WOX1-1, WOX1-2) to characterize them besides the lines of each single MYB gene over-expression (MYB12OX and pap1-D by integrated analysis of metabolomics and transcriptomics. The integrated analysis showed flavonoid-specific overaccumulation and induction of expression of stress-related genes in the overexpression plants. Abscisic acid (ABA) was not changed among Col-0 and the TF-overexpressing plants. These results implied flavonoid biosynthetic pathway has interactions to stress response mechanism, and induction of flavonoid biosynthetic pathway improves the ability of abiotic stress mitigation. As the result of abiotic stresses (drought, oxidation and salt stress) experiments, the overexpressing plants showed the stress-mitigating ability to the abiotic stresses.
Flavonoid biosynthetic pathway has the feed-forward interaction to stress response mechanism.
