Plant and Cell Physiology Supplement
Abstract of the Annual Meeting of JSPP 2011
Conference information

Mechanical simulation of stomatal movement and live imaging studies of intracellular stuructures in Arabidopsis guard cells
*Takumi HigakiKae AkitaNoriaki KondoNatsumaro KutsunaSeiichiro Hasezawa
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Pages 0225

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Abstract
We previously reported that amounts of actin microfilaments and endosomes increased in connection region of Arabidopsis guard cells in stomatal opening using live imaging and our original image processing framework (Higaki et al. 2010 JSPP metting). In this study, we observed guard cell membrane structures in connection region using Arabidopsis expressing GFP-PIP2a. Three-dimensional imaging showed that distance between guard cell membranes decreased according to stomatal opening, suggesting mechanical stress induced at the connection region in stomatal opening. We therefore performed mechanical simulation of stomatal movement by finite element method (FEM). Two-dimensional truss modeling of a guard cell based on microscopic morphometry of Arabidopsis guard cells and FEM simulation with various condition settings revealed that intensive stress induced at connection region in the displacement that mimic stomatal opening. We will discuss a possible relationship between intracellular dynamics and mechanical stress in stomatal opening.
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© 2011 by The Japanese Society of Plant Physiologists
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