Abstract
Magnesium (Mg) is an essential element for plant. Despite the importance of Mg function, the knowledge of Mg in regard to uptake and translocation manner in plants is limited. One of the reasons is lacking the available radioisotopes of Mg. In this study, we focused on 28Mg (beta-ray emitter, half life: 21h) because 28Mg can be prepared as carrier free. Nowadays, there is not any institute where 28Mg is manufactured in the world. Therefore, we produced 28Mg using an accelerator, CYRIC at Tohoku Univ. Irradiating α beam to pure aluminum target, 28Mg was produced through 27Al(α,3p)28Mg reaction. After irradiation, the Al target was dissolved with hydrochloric solution and 28Mg was purified by chromatography. Then, 28Mg was supplied to the 3-week-old rice seedlings with 6 leaves. The plants were cultured with 28Mg in a nutrient solution for 15 to 30 min. Then, the plant samples were washed with distilled water and each tissue was separated and exposed to an imaging plate (IP).The images of 28Mg showed that 28Mg was highly accumulated in basal part of the 5th leaf sheath, 20 mm in length. We will also present the feature of Mg uptake applying different Mg concentration.
