Abstract
The micronutrient zinc is essential for all organisms because it is required as a cofactor in over 300 enzymes. On the other hand, excess zinc is also toxic to cells, presumably due to competition with other biologically important ions, which in plants ultimately leads to reduced biomass leaf chlorosis, and root inhibition. To understand the effect of excess zinc on plant cells, we have performed iTRAQ based quantitative proteomics. Arabidopsis grown on MS medium were treated with high concentration zinc (300µM ZnSO4) for 0, 3, 6, 16 hr, and microsomal fractions were isolated from roots. Each fraction was used for iTRAQ analysis. About 1000 proteins including membrane proteins by zinc treatment were identified and quantified. The changed ratio of these proteins was calculated using software MASCOT. Based on the iTRAQ data, we would like to discuss mechanisms of early response proteins by excess zinc in Arabidopsis root.
