Plant and Cell Physiology Supplement
Abstract of the Annual Meeting of JSPP 2011
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Effects of the region-specific expression of RPL4D on the suppression of the abaxial-adaxial polarity defect in as2 rpl4d
*Kouji KojimaHirokazu TsukayaGorou Horiguchi
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Pages 0336

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Abstract
The ribosome plays a critical role in leaf morphogenesis in Arabidopsis thaliana. Loss-of-function mutants of ribosomal protein (r-protein) develop pointed leaves. We have isolated more than 20 mutants exhibiting such a phenotype. We found that the adaxial defect of asymmetric leaves2 (as2) was enhanced by these mutations, especially rpl4d. These results suggest that r-protein genes have a role for establishing the abaxial-adaxial polarity. But, how the ribosome regulates specific gene expression is not known. In order to assess whether the r-protein gene contributes to the activation of adaxial genes or the repression of abaxial genes, RPL4D with a target sequence of tasiR-ARF or microRNA165/166 was introduced into the as2 rpl4d double mutant. These constructs are expected to allow abaxial or adaxial region-specific expression of RPL4D. We are now investigating the effects of the region-specific expression of RPL4D on the establishment of an abaxial-adaxial polarity. We will discuss possible mechanisms of the regulation of abaxial-adaxial polarity via r-protein genes.
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© 2011 by The Japanese Society of Plant Physiologists
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