Abstract
Shoot and root meristems are continuously located at growing tips and give rise to all kinds of plant tissues. Therefore, to understand plant development, it is important to examine the mechanisms of meristem development. In Arabidopsis, it has been known that CLAVATA3 (CLV3) peptide negatively regulate the size of the shoot meristem via receptors like CLV1, CLV2, CRN, and RPK2. Moreover, Over-expression of CLV3 or CLV3/ESR-like (CLE) and treatments with CLE peptides reduce the size of root meristem and the length of root, suggesting that CLV-like signaling pathway also acts in the root. To identify the new factors regulating meristem development, we performed the clv2 enhancer screening as an index of CLE peptides-resistance phenotype of root. We identified causative mutation of one of clv2 enhancer mutants by a combination of rough mapping and deep-sequencing analysis. Here, we will report our recent results on a role of this causal gene in CLV signaling pathway.
