Abstract
The peroxisome is one of the organelles that ubiquitously found in eukaryotic cells. The peroxisome is metabolically diverse organelles with essential roles through life of the plant such as lipid metabolism, photorespiration, degenerating reactive oxygen species, and biosynthesis of plant hormone. Peroxisomes in higher plant undergo reversible functional transition depending on environmental and tissue-specific developmental manner. However, numerous novel peroxisomal proteins remained to be unidentified. In this study, we performed an in depth quantitative proteome analysis of soybean peroxisomes.
Glyoxysomes and leaf peroxisomes in the transition stage of soybean cotyledons were isolated by iodixanol density gradient centrifugation. Using iTRAQ labeling and LTQ-Orbitrap, We performed peroxisomal proteomic analysis for quantitative comparison of the peroxisomal proteins in the transition stage. Glyoxysome proteins and leaf peroxisome proteins were clearly distinguished by pattern of protein accumulation during the peroxisomal transition. We also report on exclusion of contaminated proteins and identification of novel peroxisomal proteins.
