Abstract
The moss Physcomitrella patens development starts from a germinating spore that gives rise to a filamentous structure called protonema. Bud that is arisen form protonema develops to give rise to a leafy shoot called gametohpore. After excision of a leaf from a gametophore, leaf cells facing to the dissected cells are reprogrammed and change their cell fates to protonemal cells, associated with cell cycle reactivation. We have demonstrated that E2F transcription factors are involved in the reprogramming process. To understand the roles of the E2F in the process, we performed the ChIP sequence analysis with the massively parallel sequencer to identify the E2F direct target genes. We found that the E2F bound with promoter regions of genes encoding histones and putative DNA replication factors. This suggests a conserved role of the E2F in the cell cycle regulation. In addition, the E2F bound with promoter regions of genes encoding chromatin-remodeling factors. In this meeting, we will discuss how E2F controls the reprogramming in an excised leaf.
