Abstract
To clarify relationship between cell cycle regulation and embryo morphogenesis, we focus on the role of cell cycle inhibitor(s) during somatic embryogenesis in carrot.
For isolation of KRP-homologue-genes, we design degenerated primers based on amino acid sequences in conserved CDK-cyclin binding domain. Conclusively, two cDNA fragments were amplified by RT-PCR. After 5-, 3-RACE, full length-cDNAs had been isolated for both genes (DcICK1 and DcICK2). DcICK1 and DcICK2 consist of 229 and 231 amino acids, respectively. They are highly homologous with AtKRP3 from Arabidopsis, and, motif compositions are also similar to the KRP. These genes highly express around shoot and root meristems. However, no significant change of these expressions is observed during somatic embryogenesis.When localization of DcICK1-mRNA was detected by in situ hybridization method, it is detected in whole site of globular shaped embryos or primodia of cotyledon in torpedo shaped embryo.
