Abstract
E.globulus, one of the major afforestation species is known to be difficult for vegetative-propagation. So far, it was necessary to develop novel method for promotion of adventitious root (AR) formation. First, we measured endogenous levels of several hormones. As a result, the IAA level of easy-rooting line was two times higher than that of the poor-rooting line. Next, we forcused on the cytochrome P450s. Several triazole-containing chemical compounds have previously been shown act as efficient inhibitors of cytochrome P450. A chemical library of triazole derivatives to find chemicals which have the effect of promoting AR formation was screened. Consequently, five compounds effectively promoted AR formation.
Finally, we investigated how these chemicals affected the growth of Arabidopsis thaliana. Arabidopsis seedlings were grown on agar medium containing the chemicals. One of the selected chemicals, MA65 increased the numbers of root in Arabidopsis seedlings. The amounts of endogenous IAA in Arabidopsis grown in the presence of MA65 were analyzed. The IAA content was increased by two-fold. Taken together, MA65 may increase endogenous IAA level and promotes an AR formation.
