Abstract
Plant pathogens secret a buttery of hydrolytic enzymes to degrade plant cell walls, which causes to loosen the wall and enhances the acquisition of carbon source nutrients. To avoid cell wall degradation plants produce inhibitor proteins by means of inhibiting hydrolytic activities. Inhibitor proteins against xylanase and endopolygalacturonase are well studies. Plants also secret hydrolytic enzymes such as chitinases and 1,3-beta-glucanases to degrade pathogen's walls. Subsequently produced wall fragments from pathogens are recognized as elicitors. Furthermore, a receptor protein that recognizes xylanase is proposed to trigger plant defense response. Thus, plants have evolved defense responses in apoplast against plant pathogen's attack. Here, we demonstrate plant responses to hydrolytic enzymes from Magnaporthe oryzae, in which glycosyl hydrolase family (GHF) 12 endoglucanases triggered hypersensitive response cell death in Nicotiana benthamiana leaves. Furthermore, functional analyses of GHF 12 endoglucanases are presented.
