Abstract
Sulfur is an essential nutrient for plant growth. Plants have a developed mechanism that regulates sulfur assimilation for survival under sulfur deficiency (-S). To identify the key regulator in -S response, we isolated Arabidopsis mutants showing aberrant responses to -S. The expression of SULTR1;2 sulfate transporter was visualized as GFP fluorescence in a transgenic plant expressing SULTR1;2 promoter-GFP fusion gene construct. This plant was used as a parental line for the EMS mutagenesis and the plants showed reduced GFP accumulation under -S were isolated. Previously, we reported SLIM1, a transcription factor which controls broad range of -S responsive gene expression from the analysis of these mutants. In this study, we isolated and characterized 4 slim mutants other than slim1. Accumulation of SULTR1;2, sulfate uptake activity and the contents of sulfur-containing compounds were analyzed under various sulfate concentration. We will report the different characteristics of these 4 mutants in sulfur responsibility and morphological aspects.
