Abstract
We have developed and opened a comparative predicted-proteome database, termed SALAD database (http://salad.dna.affrc.go.jp/salad/). The current version of database has provided both similarity clustering and distribution diagrams of evolutionarily conserved peptide motifs as a graphical output for predicted-proteome data of ten distinct plant species to predict some biological functions of homologous genes. We here developed a new interactive tool, termed Blasting SALAD Analysis, in this SALAD database, for users who want to use more species information than those in the current version. Using this tool, users can execute SALAD clustering analysis using an arbitrary set of protein sequences (maximum limit is 50), which have a homology with a query gene of their interest selected by a blast search. Users can select any datasets from 17 species (species specific search) or uniprot plant division dataset (wider search) for the blast search. This system is a more powerful tool for comparative analysis using paralogous or orthologous genes. Indeed, users can often get a more reliable information about tracing evolution of conserved motifs.
