Abstract
Cryptomeria japonica D. Don is one of the most important Japanese conifer species. If we can repress pollen production, we will be able to reduce risks of transgene escape from GM C. japonica into native gene pools. We attempt to generate male sterility C. japonica by introducing barnase gene driven by a male strobilus specific promoter from C. japonica.
Creations of the male sterility GM trees have been reported by using the constructs that consists of male flower specific promoter and Barnase gene. In many of these cases, disturbance of vegetative development have been also reported. For the purpose of the repression of unwanted effect of the growth, the Barnase-Barstar systems were used. Now we are trying to introduce the Barnase-Barstar constructs, that consist of male strobilus specific promoter (1009-C47-p and 1009-C96-p) of C. japonica or anther specific promoter (BoA3-p and BoA9-p) of Brassica oleracea, into C. japonica.
Acknowledgements: This work was supported in part by AFFRC, MAFF, Japan
