Abstract
Rice has perception systems of flagellin from rice-avirulent strain of phytopathogenic bateria Acidovorax avenae. To clarify the flagellin perception mechanism in rice, several domains of the flagellin were prepared and induction activity of each domain was examined. The flagellin C-terminal domain was induced the immune responses in rice, whereas the flagellin N-teminal domain, containing flg22, did not cause any immune responses. Gene expression profiling during flagellin treatment showed that expression of several genes encoding the receptor kinase were increased. Among the identified genes, Flagellin-induced Receptor Kinase 1 (FliRK1) complemented the flagellin recognition ability in fls2 mutant. Two flirk1 disruption mutants in rice lost the ability of the flagellin perception. Immuno precipitation and immuno detection using anti-flagellin antibody, showed that FliRK1 specifically interacted with flagellin. Our results suggest that FliRK1 is specific receptor of the flagellin in rice.
