Abstract
CRES-T (Chimeric REpressor gene-Silencing Technology) is a novel and powerful gene silencing system. In this system, a chimeric repressor, produced by fusion of a transcription factor (TF) to a plant-specific repression domain, dominantly suppresses the expression of target genes over the activity of endogenous and functionally redundant TFs. This system makes it possible to produce transgenic lines with strong phenotype which cannot be seen in the single loss-of-function mutants due to the existence of functionally redundant paralogs.
In this study, we have screened for Arabidopsis CRES-T lines which show abnormal phenotype in dim light aiming for the identification of TFs which directly or indirectly participate in the regulation of light responses, and also finding lines which show visible phenotype only in dim light. So far, 250 lines with abnormal phenotype were isolated in dim light. Of these, 80 lines were selected and those causal TFs (or transcription related factors) were identified. We are now performing further analyses of the selected lines in the dim and regular light conditions.
