Abstract
We are interested in stress responses during hydrogen production in Chlamydomonas reinhardtii at the genomic levels. At the first step, in order to understand genetic information including transcription and gene structures on the genome, we have developed genome database, Kyoto Chlamydomonas Genome Database (KCGD) using mating type-minus (mt-) strain C-9. In order to map the transcribed regions on the Chlamydomonas genome, full-length cDNAs derived from mRNA isolated from the cells that suffered to sulfate-limiting stress were fragmented and subjected to Illumina genome analyzer, in which 76-nucleotide sequences were determined. Of 46,920,630 total reads, 12,268,213 (26.1.%) were uniquely mapped to the Chlamydomonas genome. We have mapped short reads of 36 nucleotides on the genome, sulfate deficient-related genes and genes related to hydrogen production on the genome database KCGD. (*http://chlamy.pmb.lif.kyoto-u.ac.jp/) RNAseq data will also reveal the coding regions which may contribute to the hydrogen production or adaptation for S-deficient stress conditions.
