Abstract
Aluminum (Al) causes inhibitions of plant-growth in acid soil area and various genes change their expressions under the stress. As Arabidopsis AtGST11 gene is induced by Al stress, heavy metal stress and oxidative stress, we believe that this is a good model system to characterize the common response mechanism for these stresses. Isolation and characterization of transcription factors (TF) related to the expression of AtGST11 are performed in this study. We have already isolated 4 candidates for TF #11-1-1, #13, #11-1-3 and #43. First, candidates were confirmed their DNA binding capability to the promoter of AtGST11 by gel-shift assay. Furthermore, dual Luciferase assay was performed to estimate the effect of the 4 clones in the gene-expression of the AtGST11. The result suggested that #11-1-1 and #11-1-3 are activators and #13 and #43 are repressor. Real-time PCR showed that #11-1-1 activated the gene-expression of AtGST11 gene under Al and Cd stresses, and that #13 and #43 repressed the expression under cold and heat stresses. These TFs may contribute to the expression of AtGST11 under each condition.
