Abstract
Dynamic modification of Ser/Thr redidue with O-linked β-N-acetylglucosamine is known to be very common in nucleocytoplasmic proteins. O-GlcNAcylation has been well studied in mammalian, and more than 500 proteins have been identified to be O-GlcNAcylated. In Arabidopsis, it has two O-GlcNAc transferase (OGT), SPINDRY (SPY) and SECRET AGENT (SEC). And the double mutant of these two has shown to be lethal at embryo stage, which likely indicates that they should essentially function in overlapped and uniquely in plants. In spite of those, OGT targets remain to be found yet in plants. So in this study, we will show the occurrence of O-GlcNAcylation and characterization of the apparent O-GlcNAcylated targets at various development stages in Arabidopsis.
