Abstract
Glucosinolates (GSLs) are sulfur containing glycosides, characteristic secondary metabolites to Brassica family. GSLs are reported to function as defense chemicals against harmful insects or pathogens. GSLs are considered as a potential storage source of sulfur, because they are accumulated in seeds and their decomposition is promoted under sulfur deficiency.
In the case of Arabidopsis thaliana, it has been reported that GSLs content in seeds is the highest among the tissues and there is a difference in chemical composition of GSLs between seeds and leaves. However, the detail of GSLs content and profile during the developmental stage is not clear.
In this study, we measured the changes of 24 types of GSLs during the developmental stage of siliques, until 10 days after flowering. The total content of GSLs in siliques was not increased until 5 days after flowering and was increased from 6 days to 10 days after flowering. During this period, 4-Methylsulfinylbutyl GSL was decreased, whereas 4-Methylthiobutyl GSL and 4-Benzoyloxybutyl GSL were increased. We also analyzed aop3, one of the mutants with different GSLs profile compared to that of wild type, in the same way.
