Abstract
Production of reactive oxygen species (ROS) by NADPH oxidases has been shown to play crucial roles in plant signaling including regulation of biotic and abiotic defense responses, programmed cell death and root hair development. By heterologous expression in HEK293T cells, we showed that a respiratory burst oxidase homolog (rboh) proteins, AtrbohC and D, possess ROS-producing NADPH oxidase activity that is synergistically activated by binding of Ca2+ and phosphorylation. Ten boh genes, AtrbohA-J are present in Arabidopsis. Though genetic studies suggest functional diversity of each Atrboh, their molecular characterization still remain limited. We are attempting to perform comprehensive comparative analyses of the 10 isozymes, AtrbohA–J. ROS-producing activity and its regulation of AtrbohA-J heterologously expressed in HEK293T cells, as well as expression patterns of each isozyme will be discussed.
