Abstract
Dwarf phenotype is useful to use the limited space efficiently. Using a novel gene silencing system, called CRES-T, which could efficiently suppresses the expression of target genes, we identified a transcription factor, HR0444, which is involved in dwarf phenotype. In HR0444 CRES-T transgenic Arabidopsis, the brassinosteroid inducible gene expressions were reduced and cell elongation was remarkably suppressed in leaves and internodes. HR0444 was showed to have a repression activity and its over expression also exhibited dwarfing phenotype. HR0444 CRES-T transgenic tobacco also exhibited dwarfing phenotypes, in which leaf weight and content of total protein was increased than wild type per cultivation space. The amount of GUS protein in 35S:GUS HR0444 CRES-T transgenic tobacco was also increased than that in wild type. These results suggested that the HR0444 chimeric repressor is a useful tool for optimization of plants for plant factory.
