Abstract
In our previous study, we found that CLE1-7 encoding 12 amino acid signaling peptides repress lateral root (LR) elongation associated with the function of CLV1 receptor kinase. Regulatory components directly controlling LR elongation can be postulated downstream of the CLE-CLV1 signaling cascade, however, its function in roots has not been clarified yet. In this study, we surveyed candidates of the downstream components of CLE-CLV1 cascade, and analyzed their functions in root development.
We first conducted microarray analysis of mRNA from roots of CLE3 overexpressor plants or clv1 mutant, and compared with the wild-type plant roots. We found nine genes, which responded oppositely between CLE3 overexpressor and clv1 mutant. Among these genes, we focused particularly on three genes whose transcript levels were most significantly affected by CLE3 overexpression and/or clv1 mutation.
These three genes encoded a heat-shock protein, a putative transcription factor and a transporter family protein, respectively. Overexpressors and promoter-GFP lines for all three candidate genes were constructed. Localization and roles of these candidates in root development are being analyzed.
