Abstract
We analyzed gene expression of 9 candidates of Mg transporters in Oryza Sativa, OsMRS2, homologous transporters to AtMRS2 which are Mg transporter family in Arabidopsis.
OsMRS2 gene expression of rice plant was investigated using semi-quantitative reverse transcription-PCR and real-time PCR. As a result, most of the OsMRS2 members were expressed in whole tissue of the plant. In addition, the expression level in shoot was higher than the level in root during the vegetative stage. Then, the expression of OsMRS2 gene in root and up-ground part of the seedling under Mg deficiency was examined during 8 days of development. It was found that the expression levels of all 8 genes (one of the genes was not detected) were not induced by Mg deficient treatment during 8 days. On the other hand, gene expression level in sheath of developing leaf was increased up to about 30 times higher while the growth.
To detect histochemical localization of the OsMRS2, GUS reporter gene construct comprising the upstream region of OsMRS2 was introduced into a rice plant. The stain was observed in the sheath part of the transgenic plant and further analysis is now underway.
