Abstract
We have identified RSOsPR10 as the root specific protein induced by drought and salt treatments in rice. Expression of RSOsPR10 was up-regulated by JA and ACC, while SA strongly suppressed its expression. We conducted analyses for putative transcription factors involving RSOsPR10 expression. The results revealed the involvement of OsERF1, an ET/JA-responsive factor, in RSOsPR10 induction, indicating that there is the the ET/JA signaling pathway. OsTGAP1, encoding an elicitor-inducible bZIP transcription factor, was also induced synchronously with RSOsPR10 by salt and JA. These results suggested that RSOsPR10 expression is regulated by the complex interaction of multiple transcription factors. Because GCC-box and TGACG-motifs (as-1-like elements, TGACG (T/G)) were found around 2- to 3-kb RSOsPR10 promoter region, we are currently investigating the cis-elements for RSOsPR10 expression using the rice plants transformed with promoter (2-, 3-, and 4-kb)::GUS constructs. In addition, the transient expression system using rice culture cells by introducing promoter::LUC constructs is under establishing.
