Abstract
We successfully preserved cultured moss cells in liquid nitrogen by desiccation methods. In this study, we cryopreserved cultured rice cells by desiccation methods.
Subcultured rice cells could not survive desiccation, then the cells were precultured in MS medium containing 0.4 M sucrose to enhance the desiccation tolerance. As desiccated moss (Pogonatum inflexum) cells can be preserved for 20 weeks at 4 deg. C and 8 weeks at 16 deg. C, precultured and desiccated rice cells were also preserved at other temperatures. Precultured and desiccated rice cells died after preservation for only 1 day at 26 deg. C, in spite that 60% of the cells survived for 1 week preservation at 4 deg. C. Precultured and desiccated rice cells did not survive higher temperature treatment, though 60% of Pogonatum cells survives 80 deg. C treatment for 1 hour.
