Abstract
Magnesium (Mg) is an essential element for plants and required for a number of enzyme reactions in plants. We obtained six lines of Arabidopsis mutants from EMS mutagenized M2 population with altered Mg response to understand response mechanism of plant against Mg. They showed very short roots in high-Mg condition (14~20 mM) compared with the normal Mg condition. The phenotypes of mutants was indistinguishable between the media containing MgCl2 and MgSO4, suggesting that the phenotypes of mutants depend on Mg concentration not anions. These lines also showed a very similar phenotype in the low-Calcium condition (0.15~0.2 mM). Mg and Ca concentration in above ground tissue of mutants were measured. Concentration of Mg and Ca are lower than WT. We mapped the cause gene of this phenotype. In the result, both 2 lines have mutation on chromosome No.1. In the future, we will carry out gene mapping in detail together with physiological and molecular biological studies.
