Abstract
We performed comprehensive gene expression profiling of rice root using Laser Capture Microdissection and microarray approach. Root samples were obtained from japonica rice grown in nutrient solution for 10 days. The crown roots were divided into eight sections from the root tip to the basal zone corresponding to various stages of root development. Two individual zones were further divided into three sections corresponding to distinct root tissues. RNAs were extracted from each sample and used for hybridization using the rice microarray (Agilent). Clustering analysis showed preferential gene expression in each stage and tissue. Many DNA replication and translation related genes that maybe involved in cell division and differentiation were identified in the cell division zone. Transporter genes associated with transport and uptake of water and nutrients were observed in the root hair zone. Moreover, highly expressed genes were identified in tissues involved in the formation of root hair and lateral root. A comprehensive gene expression profile of root will provide valuable clue in understanding the gene regulatory networks of root development and morphogenesis in rice.
