Abstract
Shoot primordia of Micro-Tom, a model cultivar of tomato (Solanum lycopersicum L.), were induced from apical domes of shoot tips in liquid MS medium supplemented with 20 or 30 g/l sucrose, 0, 0.02, 0.2, 2.0, or 4.0 mg/l alpha-naphthaleneacetic acid (NAA), and 0, 0.02, 0.2, 2.0, or 4.0 mg/l 6-benzylaminopurine (BAP) at pH 5.8 on a rotary shaker at 2 rpm under 10,000 lux illuminated continuously. Multiplication of these shoot primordia was most effective in a liquid MS medium supplemented with 20 g/l sucrose, 0.2 mg/l NAA, and 2.0 mg/l BAP. The shoot regeneration from the shoot primordia was observed in static culture on MS medium supplemented with 30 g/l sucrose, 3.0 g/l Gelrite, and 1.0 mg/l zeatin under illumination at 4,500 lux for 16 h and dark for 8h. Their roots were formed from the regenerated shoots in static culture on 1/2 MS medium supplemented with 15 g/l sucrose, 3.0 g/l Gelrite under illumination at 4,500 lux for 16 h and dark for 8 h.
