Abstract
The completion of the human genome project opened the new era where various biological phenomena are elucidated with massive molecular data. Although many things have been elucidated with the massive data, the data were obtained by averaging ensembles of cells which may mask important information on a real life system. These days, many people want to analyze single-cells because heterogeneity in cell pools sometimes plays important roles in a life system. However, the analysis methods for single-cells are limited now. The next generation DNA sequencer can be used for that, however, only a part of mRNA in a single-cell can be analyzed.
We have developed a new accurate quantitative analysis method for mRNA in single-cells. It uses q-PCR coupled with a single-cell cDNA library for analyzing multiple gene expression levels in single-cells. As its ability is very high, it can analyze mRNA as small as several copies in a single-cell. We are making a tool to easily carry out the accurate multiple gene expression analysis with the method. The technology and its applications will be presented in the symposium.
