Trial Production of a Hydrostatic Pressure Vessel and the Screening of Some Barophilic Bacteria

Abstract

A hydrostatic pressure vessel was designed to isolate a wide range of barotolerant or barophilic bacteria from deep sea and various environments. This pressure vessel can withstand a pressure upto 1000 atmosphere. The seven terrestrial bacteria including isolates from soil were tested for reproduction for 3 weeks under a pressure ranging from 350 to 1000 atmospheres. A strain of bacteria isolated from tomato root was found to be more barotolerant than the others. The pressure vessel described in the current investigation may be useful for the isolation of barophilic bacteria.