Abstract
Recently, we proposed time-resolved X-ray observations of picometer-scale slow Brownian motions of individual protein molecules in aqueous solutions. In this new single molecular detection system, which we call Diffracted X-ray Tracking (DXT), we observed the rotating motions of an individual nanocrystal, linked to the specific site in individual protein molecules. Therefore, DXT can monitor dynamics of the individual molecules or specific sites in individual single molecules. Now, we observed individual DNA molecules, myosin head molecules, denatured proteins, and functional protein membranes. The most important techniques for DXT are both the size and crystallization of the nanocrystals that are labeled with single molecules. Here we introduce two fabrications of the labeled nanoparticles or nanocrystals.
