Abstract
To perform basic research on infective wounds, we need simple and persistent infective models. When a liquid medium mixed with Staphylococcus aureus was injected subcutaneously, infection resolved within 3 days. We applied the semi-liquid medium in addition to agar, which produced favorable results. So, we present this simple model. When we injected the semi-liquid medium including S. aureus (5 × 108 units / ml), colony formation occurred within 3 days as agar was an obstacle to cellular infiltration. On Day 5, the number of colonies increased and an abscess formed. The size of the abscess depended on the number of S. aureus. Infections were observed on the abdomen, back, and head. Infection persisted for 9 days. Taken together, this model is simple and useful, involving a simply prepared semi-liquid medium, which produced a one-week, persistent infection.
