Evaluation of the usefulness of immunocomplex capture fluorescence analysis（ICFA） in a lymphocyte crossmatch

Abstract

【Objective】The results of a lymphocyte crossmatch test obtained by histocompatibility laboratories are discrepant, because the assay procedures and evaluation criteria differ. In the present study, we examined the usefulness of the newly developed immunocomplex capture fluorescence analysis (ICFA) method for a lymphocyte crossmatch．
【Methods】Serum and whole blood samples were obtained from 12 subjects. They were subjected to complement-dependent cytotoxicity crossmatch (CDC), flow cytometry crossmatch (FCXM), and ICFA methods at 3 different laboratories, and then the results were compared．
【Results】The results for 11 of the 12 subjects in regard to ICFA class Ⅰ and Ⅱ were the same among the laboratories. In contrast, the results of only 5 regarding FCXM-T and 8 for CDC-T were the same among the 3 laboratories．
【Conclusion】Of the 3 crossmatch methods investigated, ICFA had the highest rate of concordance among the 3 laboratories.