Abstract
【Objective】The sensitivities of antibody testing using single antigen beads on the Luminex platform and flow cytometry crossmatch (FCXM) testing are higher than the sensitivity of the traditional estimation of complement-dependent cytotoxicity (CDC) for the detection of anti-human leukocyte antigen (HLA) antibodies. Therefore, these novel techniques are considered important for the prediction of graft survival before kidney transplantation. Here, we examined the usefulness of FCXM and immunocomplex capture fluorescence analysis (ICFA) in 188 kidney transplant candidates.
【Results】The results showed that permeabilization with a solvent was required in FCXM for immunostaining, but not in ICFA. On comparison of the results of FCXM with those of ICFA in terms of the presence of HLA antibodies and histopathological characteristics, we found that the results of FCXM showed a correlation of 100% to those of ICFA for HLA class I antibody and of 95% for HLA class II antibody.
【Conclusion】The results of the present study showed that FCXM and ICFA were useful in clinical practice because they required only a small amount of blood sample from the donor and did not necessitate the extraction of lymphocytes. Furthermore, the results of ICFA showed a good correlation with those of FCXM. These findings suggest that ICFA and FCXM can be useful tools for the preoperative evaluation and postoperative monitoring of HLA antibodies.
