Abstract
An AFLP-based genetic linkage map of diploid Ipomoea trifida was constructed using the F1 mapping population from the cross between two lines, ‘0431-1’ and ‘Mx23-4’, as the maternal and paternal parents, respectively. Based on the “pseudo-testcross design” for analyzing F1 populations from heterozygous parents, the genotype data of the AFLP markers were split into maternal and paternal datasets, and separate genetic linkage maps were constructed. The maternal map was comprised of 618 markers on 17 linkage groups with a total map length of 910.0 cM per haploid, which approximately corresponds to one-sixth of the total map length of the hexaploid sweetpotato map. The paternal map consisted of 163 markers mapped on 15 linkage groups covering a total map length of 503.3 cM. The genetic linkage map presented here is the first step to construct a diploid-level reference linkage map which will be useful for analyzing the allelic relationship(s) of gene(s) controlling important agronomic traits.
