Exploration and DNA analysis of Local Citrus Genetic Resources on Ambon Island of Indonesia

Abstract

Exploration of local citrus genetic resources grown on Ambon Island, Maluku, Indonesia was conducted in June and July 2014. Among the 28 accessions investigated, three accessions purchased at a local market were from Seram Island close to Ambon Island. The remaining 25 accessions were local citrus genetic resources grown on Ambon Island. According to the morphological traits, the citrus collected could be grouped into lime (Citrus aurantifolia), purut (C. hystrix), calamondin (C. madurensis), pummelo (C. maxima), sweet orange (C. sinensis), and mandarin (C. reticulata). Cleaved amplified polymorphic sequence (CAPS) analysis of the accessions was conducted, and precise accession identification was possible based on the results of CAPS analysis in conjunction with the morphological traits. The results of CAPS analysis revealed that genetic diversity exists in accessions with the same name on Ambon Island. The genotypes of some accessions resembled to lime, purut, calamondin, sweet orange, and mandarin were differed from those of control species. In contrast, genotypes of all four “Lemon Bali” accessions were identical to those of pummelo. These results indicate the individuality and diversity of citrus genetic resources grown on Ambon Island.