Abstract
Young leaves in Cinnamomum species were used as materials. Preparations for electro-microscopical observations were made as follows
1) fixation; osmic acid-phoshoric buffer mixture containing sugar at the rate of 2%
2) dehydration; ethyl alcohol and propylene oxide
3) imbedding; epon resin
4) electronic staining; aceticuranium and causticlead
The authors observed the microscopic structures of nucleus, nucleolus, nuclear membrane, cell wall, cell membrane, cytoplasm, mitochondria, endoplasmic reticulum, Golgi's apparatus and chloroplast. In particular, Golgi's apparatus were ob- served in the peripheral regions of the oil- vesicle cell.
Osmium-stainable granules in the chloroplast of Cinnamomum plants were considerably large as compared with granules of other aromatic plants (cf. YASUNAGA and YOSHIDA, 1973) . In sections most of these granules took a spherical shape, which suggests that they are liquid. Their electron density was not so high.
There were other kinds of osmium-stainable coherent masses in the cytoplasm. They were highly electron-dense, presumably viscous, irregular in shape, and pressent in a majority of cells.
The essential oil in the vesicle in fusible with ethyl alcohol and propylene oxide. There was found no complicated structure in any vesicle.
In conclusion, there are three kinds of oil-like substances in the plant, i. e., osminm-staihable granules in chloroplats, osmium-stainble coherent masses in the cytoplasm, and essential oil in vesicler. These substannces are supposed to be different in their chemica components from one another.
