Concentration of cyclic AMP in body fluids in acute stage of subarachnoid hemorrhage due to ruptured intracranial aneurysm
1980 Volume 2 Issue 3 Pages 291-298
Details
1980 Volume 2 Issue 3 Pages 291-298
There are a few reports which indicate a close relationship between cerebral vasospasm in subarachnoid hemorrhage (SAH) and metabolic impairment of cyclic adenosine 3', 5'-monophosphate (cyclic AMP) in the vascular smooth muscle.
Ten patients with SAH due to ruptured intracranial aneurysm admitted to our hospital within 5 days after the onset were analyzed concerning the correlations between the concentrations of cyclic AMP in body fluids, and clinical grading of SAH (Hunt & Hess's) and degree of vasospasm on angiograms, respectively.
Body fluids were collected into syringes moistened with 0.5 M. disodium ethylene-diaminetetraacetate and centrifuged, and supernatants were analized for the concentrations of cyclic AMP applying a competitive protein binding method (Radiochemical Co.). The body fluids consisted of plasmas obtained from the antecubital vein (AVP), the internal jugular bulb (IJP) and the femoral artery, and cerebrospinal fluids. Cerebrospinal fluids were obtained by lumbar puncture or from the tube of continuous ventricular drainage.
In the early stage of SAH, cyclic AMP concentration in AVP showed an increase over the normal range (8-18 picomolese/ml) in 7 cases. This elevation of cyclic AMP concentration returned to the normal range within 10 days after the onset. In cases without vasospasm, the level of cyclic AMP concentration in AVP had a tendency to take higher level than in cases with vasospasm within 4 days after the onset of SAH.
The cyclic AMP concentration in CSF showed scattering values in each case, but showed a gradual increase during 2 weeks after the onset in majority of cases.
IJP had a tendency to take the highest concentration of cyclic AMP among plasmas obtained simultaneously in each patients. This phenomenon might suggest the release or leakage of cyclic AMP from the brain. But, whether the origin of cyclic AMP is the brain tissue or the vascular smooth muscle, has not yet been clarified.
