Abstract
The experimental study presented was undertaken to evaluate the role of platelet function contributing the cerebral microcirculatory disturbance during the post-cerebral ischemia, and to examine effects of antiplatelet agent, ticlopidine, on this condition.
Following Pulsinelli's method, rats were subjected to global cerebral ischemia by means of temorary clips on both common carotid for fifteen or thirty minutes after previous ligation of both vertebral arteries. The animals were assigned to three groups by administration of the drug as follow; 1) intact control group, 2) ischemic group without drug, 3) ischemic group with 100 mg/kg of ticlopidine given orally three hours before insult. The morphological changes of platelet were observed by scanning electron microscope and the platelet aggregability to ADP were recorded on each group at five minutes and thirty minutes after recirculation. The degree and distribution of no-reflow phenomenon after reperfusion was assessed by means of carbon black perfusion method in serial coronal section.
Compared with the intact control group, ischemic animals without ticlopidine showed significantly increased aggregability of platelet and increased numbers of activated from of platelet during their post-ischemic period. This group also had impaired reperfusion after ischemia on carbon black perfusion method.
While, in ischemic group treated with ticlopidine, platelet aggregability and number of activated form of platelet were significantly decreased and impaired reperfusion seemed to be also improved. Ticlopidine improved survival rate after fifteen minutes of cerebral ischemia but did not in cases of thirty minutes of ischemia.
