Abstract
This fixed strain was discovered accidentally in our laboratory during experimenting the intravenous infection with various kinds of rabies virus. The experiment was made on the rabbits and carried out with passed and fixed viruses. In April, 1927, a cat kept by a merchant in the central part of Kyoto City (Shimmachi Anegakoji-agaru) became missing. When she was found at home after a month, she was fretful, had no appetite and staggered, and crouched in a dark corner; three persons of the family who passed her were bitten on the 1 egs and hands; the wounds, which were not deep, were painted with iodine; the cat died under paralysis 3 days later. As there was an epidemic of rabies in Kyoto at that time and the preventive measure had been decreed by the police, the victims visited our laboratory; the vaccins were given to them immediately and the dead cat was sent to us at the same time, and the body was dissected; there were no marked changes with the naked eye anywhere, no foreign bodies in the stomach.
The brain of the cat was inoculated into the rabbits and the reaction was positive. The transmission experiments on rabbits and guinea pigs were carried out successively, and at about the 30th generation the strain was fixed at the 8th day. The strain has passed through 268 generations at present. It is necessary to test the immunizing effect of this virus for antirabies vaccine, as well as to conduct the histological studies with the brains of animals suffering from the disease uninfluenced by the cerebral trauma. We will be glad to send yon the virus at any time if requested. We are awaiting your criticism.
