Abstract
Since several years, the author has been studying on the complement fixation reaction with various materials containing various pathogenic agents as antigens. During the course of his studies, he discussed numerous aspects to be considered for serologic diagnosis; especially, he pointed out the necessity of the considerations on the following:
1) the purification of antigens,
2) presence or absence of inhibitory substance for antigen-antibody reaction in the antigens,
3) the normal components other than components of lipin series,
4) considerations on the mode of reaction.
The author and his co-workers investigated the field dimension of antigen-antibody by means of the two dimension method, therefromm they pointed out that, with the presently prevailing method, that is a testing technique on a point or along a line of a fixed antigen-antibody ratio, any case which should demonstrate a positive reaction may come out as negative as the result of the effect of the so-called zone phenomenon. They also claimed that the judgement on the grade of antibody production should be made at the optimal ratio of antigen and antibody, because an erroneous judgement might be given in case it was estimated by the antibody titer of the serum.
