Abstract
We tried to make clear the mode of action of HCl-Cysteine and Glutamic acid on the phage-hostcell system, and gained several interesting results. (See P. 130)
1) HCl-Cysteine inhibit a reaction necessary for the production of active phages. Inhibition of Virus growth was obtained when HCl-Cysteine added up to 8 to 11 minutes after infections Removal of HCl-Cysteine from the phage infected coli early in the course of latent period, namely 8 minutes, permit phage liberation. And the burst sizes of phage decreased progressively with lengthning of the interval between infection and removal of HCl-Cysteine.
When removal of HCl-Cysteine from the phage host cell system 14 to 25 minutes after infection, the liberation of phage was found and burst size of phage were as large as control.
2) In nephelometric examination, HCl-Cysteine failed to inhibit lysis of phage infected host cell, but HCl-Cysteine M/6000/ml and M/3000/ml delayed the beginnings of lysis curves.
In these lysis increasing of phage were could not found.
3) Glutamic acid or Aspartic acid was found to prevent HCl-Cysteine inhibition of phage multiplication. Precultivation of host cell in synthetic medium countaining Glutamic acid or Aspartic acid permits phage multiplication to occure in spite of HCl-Cysteine.
And these cases the burst sizes of phage were about 1/2 compaire with control.
While phage, HCl-Cysteine and Glutamic acid (or Aspartic acid) added simultaneously to the culture of host cell in synthetic medium, the burst sizes were smaller than above mentioned results.
DL-Tryptophane or L-Methionine could not prevent the inhibition of phage multiplication by HCl-Cysteine.
