Abstract
1. The production of the neutralizing antibody as well as the antihemagglutinin were investigated with the course of the intracutaneous inoculation of vaccinia virus in rabbits, comparing with the histomorphological changes in the inoculated loci with the consideration of their primary and secondary infections.
2. The neutralizing antibody reaction is more sensitive than the anti-hemagglutination reaction both in the primary and the secondary infected rabbit, although the course of the changes are nearly equal as a whole with each other.
3. The first appearance of the neutralizing antibody and the antihemagglutination reaction was noticed since the sixth day of the primary inoculation, slightly later than the plasma cell reaction in the infected skin (4th day). On the 8th day, there appears a hemorrhagic reaction in type of the Arthus-phenomenon in the inoculated skin, in accordance with which the titer of both antibodies fall transitorily, followed by the abrupt rise there after.
4. The secondary inoculation was given to those rabbits, by which the local changes in type of the Arthus phenomenon (so-called rapid reaction in Piquet's concept) appeared promptly, accompanied with the fall of the titers of antibodies. However, in this case the recovery of the antibodies was reached since the third day. Moreover, on the 7th day the plasma cell reaction appears in the infected locus with the additional formation of the antibodies in that rabbits.
5. From those transitory fall of the antibodies (neutralizing and anti-hemagglutination antibodies) accorded with the appearance of the reaction in type of the Arthus phenomenon and from the simultaneous disappearance of the viremia, we can assume an antigen-antibody reaction as a basis of those reactions in the inoculated rabbits.
