Abstract
The immunized rabbit serum against the supernatant fluid of the infected larval blood was injected to the pupae and then the challenge by the virus was performed after the varying intervals, however, the passive immunity could not be reccegnized.
After the virus inoculation, this serum was injected to the pupae st varying periods and times but the serum therapy was unsuccessful.
As reported before, when the virus is heated at 50 or 60°C for 20-30 minutes, almost all of the activity is lost. The pupae were heated at 50°C for 15 minutes just after the inoculation of the each decimal diluted virus solution, but the heat therapy was unsuccessful in the case of the inoculation of the marked diluted virus solution.
Penicillin or streptomycin solution was mixed with the equal volume of the virus solution and this mixture was maintaiaed at room temperature for 30 minutes and thereafter the inoculation of the mixture was perfomed but the decrease of LD50 or the elongation of the latent perod could not be recognized.
The immnization was effective when the vaccine made from the infected larval blood was inoculated.
