Abstract
Electrophoresis of chicken egg white was carried out by means of the Tiselius' apparatus (Type HT-A, Hitachi made) with barbituric buffer of pH 8.6 and ionic strength of 0.1, observations being made of the alterations of relative concentration of each fraction according to egg-species, incubation-period and inoculation of bouillon or vaccine-virus.
1. There were significant differences in each alteration of the fractions F, A2, A1+A2 and C1 in comparing the egg white of the White Leghorn with those of the Rhode Island Red and New Hampshire.
2. There were also significant differences in the alterations of each fraction in comparing the White Leghorn's egg white with those incubated for 5, 10, 12, 13 and 15 days. At an early stage of incubation, the alterations of the fraction A were especially recognized and at a later stage of incubation those of fraction C.
3. The 10 day-incubated White Leghorn's eggs were divided into two groups. Each egg of the first group was inoculated with 0.05cc of bouillon (pH 7.4) and each of the second group with the chorioallantoic membrane of vaccine-virus diluted to 10% with bouillon (pH 7.4). On the 2nd, 3rd and 5th day after the incubation-namely on the 12th, 13th and 15th day from the time of incubation-the fractions of both groups were compared. On the 5th day the increasing of the fraction G was recognized in both groups and on the 2nd and 3rd day the alteration of the fraction G showed that in the eggs inoculated with bouillon and vaccine-virus there was a dissimilarity in comparison to the time elapsed.
