1957 Volume 7 Issue 4 Pages 271-273
In the first report, the studies on purification and concentration of GDVII virus by means of hemagglutination procedure were described. However, that the concentration of the virus by this procedure did not produce satisfactory yields of virus because of hemolysis appearing in the process of concentration of the virus by elution from erythrocytes. The purpose of the present paper is to report the research on more effective method by means of adsorption to and elution from the red cells stroma which are capable of adsorption of virus.
In the first step, the virus was adsorbed to the human O Group erythrocytes, and was eluted from erythrocytes by saline solution in the volume which did not occur hemolysis, and finally the virus eluted into the saline solution was adsorbed to and eluted from the twenty per cent stroma suspension. The results obtained indicated that the concentration procedure of virus by stroma was more effective than that of erythrocytes for the removal of total nitrogen in the final product, i.e. the former was able to concentrate the virus in more approximately 1×106 hemagglutinating units per mg N, and 1000-fold LD50 per mg N than that of the original materials.
